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Unformatted text preview: MCB 160L Overview Forward genetics : Define a phenotype and screen or select for mutations that generate that phenotype, starting with a random mutant collection ( Arabidopsis-gametogenesis, embryonic phenotypes; Drosophila-specific gene expression patterns).mutagen = mobile DNA elements. Goa l-Move from knowledge of the phenotype to identification of the gene. Reverse genetics : Start with a specific gene, identified at the molecular level. Goal- Create or identify mutations in that specific gene in order to study the phenotype produced when gene function is reduced or removed. ( C. elegans RNAi to produce a phenocopy of mutation; S. cerevisia (yeast) targeted knockout by homologous recombination) Mapping the location of genes: Analysis of segregation and linkage to determine chromosomal location; 3-pt cross; molecular and bioinformatic tools Molecular genetics tools: DNA isolation, restriction mapping and agarose gel electrophoresis, plasmids and bacterial transformation, subcloning and genetic engineering, PCR (as a tool for construction and analysis of DNA sequences, as a method to analyze genotypes obtained from small tissue samples, as a genomics tool) Use of Model Organisms Central principles and mechanisms are conserved across phyla.study simple systems to gain information to apply to more complex questions Requirements: (1) rapid development (2) short...
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This note was uploaded on 04/04/2010 for the course MCB 160L taught by Professor Kiger during the Spring '08 term at UC Davis.
- Spring '08