Lec 2 MCB 160L F09 slides

Lec 2 MCB 160L F09 slides - w p[w+ Gal4 ]; + ; TMS, Sb p[...

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UGA Intron 3 is not removed; translation hits STOP in intron No transposase activity
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http://engels.genetics.wisc.edu/Pelements/fig3.html The ends of the gap can also be repaired by non-homologous end joining…no P reintroduced
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# p[w+] copies in genome Color of cell in eye two or more dark orange to red one (original #) orange none white
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Cross 1.0 Females w p[w+ Gal4] ; + ; + ; + x males w ; + ; TMS, Sb p[ 2-3] ; + w p[w+Gal 4] + + + Y + LVM + Females
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Unformatted text preview: w p[w+ Gal4 ]; + ; TMS, Sb p[ 2-3] or LVM ; + w + + + + Males w p[w+ Gal4 ]; + ; TMS, Sb p[ 2-3] or LVM ; + Y + + + + Cross 1.1 Females y w ; + ; + ; + x males y w + + + See if you can predict the expected genotypes/phenotypes if: (1) no transposition occurs (Mendelian segregation) or (2) if the P-element transposes to an autosome in the male germline Experimental cross (1.1a-c) Control cross (1.1d)...
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This note was uploaded on 04/04/2010 for the course MCB 160L taught by Professor Kiger during the Spring '08 term at UC Davis.

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Lec 2 MCB 160L F09 slides - w p[w+ Gal4 ]; + ; TMS, Sb p[...

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