Exam 1 Study Guide - Review Sheet, Exam 1 By: Brett...

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Review Sheet, Exam 1 By: Brett Schofield WARNING: This is not all of the information that you will need to know, but it is a good guide for your studying. Some of the details have been left out for the sake of brevity. Nuclear Transport: Small proteins (like Ran) and other molecules can diffuse through the Nuclear Pore Complex. Large proteins and protein complexes must go through active transport: o Import – NLS containing cargo binds to importin , which binds to importin . This import complex can then enter the nucleus via the Nuclear Pore Complex. Once in the nucleus, Ran-GTP out-competes the NLS containing cargo, and the importin + Ran-GTP complex leaves the nucleus. o Export – NES containing cargo binds to exportin, which also cooperatively binds Ran- GTP. This three-part complex moves to the cytoplasm, where the GTP is hydrolyzed to Ran-GDP, which causes the entire complex to fall apart. Exportin returns alone. Ran-GTP/GDP gradient is formed by the presence of Ran-GEF inside the nucleus (attached to chromosomes), and Ran-GAP in the cytoplasm. Secretory Pathway (vesicle mediated transport): Proteins are co-translationally imported into the ER. They are sorted into buds which are pinched off the ER using the COPII coat complex. These vesicles travel to the Golgi, where they fuse together to form a cis-Golgi. These proteins then travel through the Golgi by cisternal maturation (accomplished by both retrograde [using COPI coated vesicles] and antrograde transport of Golgi resident proteins). From the trans-Golgi, proteins are sorted into clathrin- coated vesicles and moved to their final destination (either an organelle or plasma membrane). Vesicles contain a number of factors that allow them to be properly targeted and to mediate
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This note was uploaded on 04/05/2010 for the course MCB 104 taught by Professor Urnov during the Spring '09 term at University of California, Berkeley.

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Exam 1 Study Guide - Review Sheet, Exam 1 By: Brett...

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