Problem Set 2
MCB 104 – Section 105/106
1.
What is the key distinction between a cell
strain
and a cell
line
? What are the
advantages and disadvantages of using each for experiments?
2.
How can you experimentally change the sub‐cellular localization of a protein?
3.
Consider the accompanying pedigree of a rare autosomal recessive disease, PKU.
(Griffiths et al., Chapter 2, Q: 42).
a.
List the genotypes of as many of the family members as possible.
b.
If persons A and B marry, what is the probability that their first child will have PKU?
c.
If their first child is normal, what is the probability that their second child will have
PKU?
d.
If their first child has the disease, what is the probability that their second child will
be unaffected?
(Assume that all people marrying into the pedigree lack the abnormal allele.)
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4.
One of the flow cytometers on the 4
th
floor of LSA has the excitation lasers and
emission filters given in the table below. Each emission filter can only be used with
the excitation laser to its left. The excitation and emission spectra of five fluorescent
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 Spring '09
 URNOV
 cell biology, PKU, Green fluorescent protein, Flow Cytometer, fluorescent proteins, excitation laser

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