Jakob_Quiz2a Key

Jakob_Quiz2a Key - MCB104Quiz2 Section105 Name 1A(2points.....

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MCB 104 – Quiz 2 Name: ____________________________ Section 105 1A (2 points). You are studying a hermaphrodite C. elegans worm with a gain of function mutation in let‐60(Ras). What would be the phenotype of this worm if you used a laser to destroy the anchor cell early in development? Why? Multivulva. In a let-60 gain of function worm the pathway promoting vulval fate is constitutively turned on, independent of the EGF/EGFR signal. Therefore it doesn’t make a difference if the AC is present or not. 1B (2 points). The anchor cell normally lies above the P6.p cell. How would relocating the anchor cell next to the P4.p cell in a wild‐type hermaphrodite affect Pn.p cell fates and what phenotype would the worm have? The P4.p cell would adopt a primary fate and P3.p and P5.p would become secondary. These cells would develop into a vulva, although it probably would not connect to the gonad and therefore me non-functional. 2 (6 pts). You have isolated five neurospora mutants (A‐E) that fail to grow in the absence of vitamin A. You make the following double‐mutants by fusing two haploid neurospora
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Jakob_Quiz2a Key - MCB104Quiz2 Section105 Name 1A(2points.....

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