Jakob_Quiz2b Key

Jakob_Quiz2b Key - MCB104Quiz2 Section106 Name...

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MCB 104 – Quiz 2 Name: ____________________________ Section 106 1A (2 points). What is the phenotype of a wildtype hermaphrodite C. elegans worm in which you have used a laser to destroy the anchor cell early in development? Why? The worm will be vulvaless. The EGF signal from the AC is required to drive P6.p into the vulval fate. In the absence of EGF all Pn.p cells adopt the tertiary fate. 1B (2 points). The anchor cell normally lies above the P6.p cell. How would relocating the anchor cell next to the P7.p cell in a wild‐type hermaphrodite affect Pn.p cell fates and what phenotype would the worm have? The cell closest to the AC adopts the primary fate and adjacent cells adopt the secondary fate. In this worm the P7.p will be primary and P6.p and P8.p will be secondary. The worm will still develop a vulva, although it may not connect to the gonad properly. 2 (6 pts). You have isolated five neurospora mutants (A‐E) that fail to grow in the absence of vitamin A. You make the following double‐mutants by fusing two haploid neurospora into a heterokaryon, and test for growth on minimal media (lacking vitamin A). The “&”
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  • Spring '09
  • URNOV
  • anchor
cell, minimal
media, C.
elegans
worm, Hermaphrodites
 
 
 
 
 Males
 dpy/+, F1
progeny

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