Leslie_Feb3 - 3c. Under what conditions will LAS be...

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MCB 104: Week 2 Practice Problems Section 103/112 1. You are trying to look two membranes separated by less than 100 nm under a light microscope. This is causing you such difficulty. Why is this the case? What other technique could you use? 2. You are interested in studying the LAS protein in mermaid cells. First, you wish to determine where in the cell this protein localizes. You have ordered live mermaid cells on the internet and are maintaining them in culture. You have the choice of attaching GFP to your protein of interest, or using an antibody directed to this protein. What do you choose? 2b. If you were instead sent fixed mermaid cells, how would this change your approach 3. You determine that LAS is a nuclear protein of 100 kDA. Assume mermaids nuclear pore complexes are identical to humans. What must LAS contain to direct its movement from the cytoplasm to the nucleus? 3b. What needs to bind LAS in order to move this protein from the cytoplasm to the nucleus?
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Unformatted text preview: 3c. Under what conditions will LAS be released into the nucleus? 4. With an eventual goal of cloning his roommates cat, Harry takes a sample of the cats tissue to establish a primary cell culture. He prepares the cells properly and provides the cells with rich media. He checks on his cells, and notices many of them are dying. How can he salvage his cell line? Unless these cells undergo an oncogenic transformation, what will occur? 5. Consider three loci E, F & G The following cross is constructed: EEFFGG x eeffgg (P) => EeFfGg x eeffgg (tester) ( F1 ) which produces the following phenotypes and counts among 1,000 F2 offspring: E F G efg 370 E F g efg 8 E f G efg 37 E f g efg 95 e F G efg 85 e F g efg 43 e f G efg 12 e f g efg 350 Are these genes linked? If so, draw a map of these genes showing distances. Determine if interference is present....
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This note was uploaded on 04/05/2010 for the course MCB 104 taught by Professor Urnov during the Spring '09 term at University of California, Berkeley.

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Leslie_Feb3 - 3c. Under what conditions will LAS be...

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