Scott_Handout2 - 2/8/2010 MCB 104; Handout #2 1....

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2/8/2010 MCB 104; Handout #2 1. Anterograde transport: there’s not a ton of memorization in this class, but you should know the order in which a secreted protein passes through the ER, and various golgi cisternae in anterograde transport. 1A. In which steps is the protein transported by vesicle? 1B. In which steps is the protein transported by cisternal maturation? 1C. How are proteins that function specifically in the Golgi and ER recycled? 1D. Describe two experimental techniques that you can use to monitor the progression of a protein through anterograde transport in the secretory pathway. 2A. In the high-throughput screen for secretory pathway mutants in yeast, which cell would have the highest density: wild-type cell at 25C (permissive temp), wild-type cell at 37C (restrictive temp), sec mutant cell at 25C, or sec mutant cell at 37C? 2B. This high throughput screen identified 5 classes of mutants: Cytosol, ER, ER-to- Golgi, Golgi, Secretory vesicle. If the following pairs of double-mutant yeast cells were
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Scott_Handout2 - 2/8/2010 MCB 104; Handout #2 1....

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