06_CPG Questions

06_CPG Questions - place to start might be the references...

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Discussion 6: Central Pattern Generators MCB 167 Spring 2010. For Discussion Section Mar 12. Reading Wyart C et al, "Optogenetic dissection of a behavioural module in the vertebrate spinal cord." Nature 461:407-10. 2009. Overview This paper uses optogenetic techniques to identify a specific class of neurons that provide input to the CPG regulating forward swim in the zebrafish. This is a methods-heavy paper. The authors first express the light-gated channel LiGluR in specific subsets of neurons. They then activate these neurons with light and measure the behavioral output. From this they infer which neurons are involved in the behavior. You will probably need to do some background reading in order to fully understand the techniques and to answer the discussion questions. A good
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Unformatted text preview: place to start might be the references cited in the paper. Questions Please answer the following questions in 1 paragraph each. Bring your written answers to section. We will use them as the basis of discussion, and you will turn them in at the end of section. Q1. How does LiGluR work? Specifically, what happens to a neuron expressing this transgene when it is photostimulated? Q2. How does the UAS/Gal4 system work? How can it be used to express a transgene in a specific subset of neurons? Q3. Summarize the evidence from this paper suggesting that the Kolmer-Agduhr neurons drive the swim CPG. It was already known that ablating these cells reduces spontaneous free swimming. How does this paper advance our understanding of the system?...
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This note was uploaded on 04/05/2010 for the course MCB 167 taught by Professor Feldman during the Spring '10 term at Berkeley.

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