lecture24 - Coupling of Transcription and Translation in E...

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Coupling of Transcription and Translation in E . coli
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Genes for Amino Acid Biosynthesis enzymes required for synthesis of particular amino acids generally are clustered into operons most are regulated by attenuation of transcription in response to levels of amino acid levels trp operon is regulated by both transcriptional repression and attenuation of transcription
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Regulation of Trp Operon mRNA from this operon has half-life of 3 min. transcription initiation regulated by trp repressor -at abundant [trp], trp repressor binds trp -complex binds to operator region and blocks transcription transcription attenuation also sensitive to [trp] in cell -after repression is relieved -allows for 700 fold variation in rate of synthesis
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Trp Operon
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Trp Leader 162 nt sequence containing 4 regulatory signals -- sequence 1 encodes short open reading frame = leader peptide (trp L) that contains 2 trp codons -key for sensing trp levels -- sequences 2 and 3 are complementary and can form secondary structure that does not cause attenuation -- sequences 3 and 4 can form secondary structure (GC rich hairpin followed by Us) that can cause attenuation
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lecture24 - Coupling of Transcription and Translation in E...

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