lecture12 - Protein/DNA Interactions Catalytic -DNA...

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Protein/DNA Interactions Catalytic --DNA dependent DNA polymerases (DNA replication) --DNA dependent RNA polymerases --Many of proteins involved in replication and transcription --DNases (endo- and exonucleases) --Restriction enzymes --Topoisomerases --many other DNA modifying enzymes (ie. kinase, phosphatase, ligase, methylase) Regulatory --proteins that enhance or activate process --proteins that decrease or repress process --proteins involved in spatial alterations of DNA (histones) --proteins that carry other proteins to DNA 1.
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Degree of Specificity Specific • Protein recognizes specific sequence on DNA or RNA ex. RNA polymerase holoenzyme, restriction enzymes, certain transcriptional activators and repressors Non-Specific Does not involve recognition of specific sequence ex. DNA ligase, RNA polymerase core enzyme 2.
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Nucleic Acid Interactions Does an interaction exist? Is the interaction specific? What region of the DNA or RNA is recognized? Which nt residues are required for contact with the protein? What region of the protein is binding to the DNA or RNA ? What is the detailed structure of the complex?
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This note was uploaded on 04/06/2010 for the course BCH BCH 453 taught by Professor Heminway during the Spring '10 term at N.C. State.

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lecture12 - Protein/DNA Interactions Catalytic -DNA...

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