lecture22 - What is required for translation? mRNA tRNAs...

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What is required for translation? • mRNA • tRNAs • tRNA synthetases • amino acids • ribosomes • peptidyl transferase • protein factors
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Ribosomes Eucaryotic Procaryotic 80S 70S 5s rRNA 28S rRNA 5.8S rRNA ~49 proteins 5s rRNA 23S rRNA ~34 proteins 16S rRNA ~21 proteins 18S rRNA ~33 proteins 50S 30S 60S 40S WHY DO COMBINED SUBUNITS HAVE S VALUE LESS THAN THE SUM OF EACH COMPONENT? HOW WOULD THE SUBUNITS AND WHOLE RIBOSOME RUN ON A GRADIENT?
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Ribosomal Proteins Procaryotic Eucaryotic 50S subunit 60S subunit L1 - L 34 L1 - L49 30S subunit 40S subunit S1 - S24 S1 - S33 MW of proteins vary from 6,000 to 75,000 Required for structural integrity and enzymatic rxns during translation
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Procaryotic Ribosomal RNA Structure Model 5’ 3’, 1542 nt
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Model of E . coli Ribosome Cleft
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Reconstitution of Functional E . coli Ribosomes Masayasu Nomura developed method to reconstitute functional ribosomes from constituent parts • purified S1- S21 and 16S rRNA can form native 30S subunit • purified L1-L34 and 23S rRNA+ 5S rRNA can form 50S subunit provided 30S subunit is present
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• highly conserved cloverleaf • G at 5’ end and CCA at 3’ end • contains modified bases - pseudouridine ( ψ ) - inosine (I)
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lecture22 - What is required for translation? mRNA tRNAs...

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