lecture17 - Factors Involved in Transcription Initiation...

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TFIIA Binds to TFIID; contains several subunits TFIIB Interacts with RNA Polymerase II TFIID Multisubunit initiation factor; recognizes TATA Box --contains TBP and various TBP-associated factors (TAFs) TFIIE Interacts with RNA Polymerase II TFIIF Contains two subunits RAP74 and RAP30 (some homology to E. coli σ factor) --may bring RNA Pol II to assembling transcription complex TFIIH Binds to transcription complex; has kinase activity TFIIJ Binds to transcription complex --required for recognition of TATA box and initiation of transcription for most class II genes Factors Involved in Transcription Initiation Complex
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1) Binding of TFIID to region extending upstream of TATA Box -- TBP recognizes and binds to TATA Box in minor groove; **** the only basal transcription factor that makes sequence-specific contacts with DNA -- TAF s are TBP-associated factors; at least 8 --interact with TBP and other TFs --can help TBP position correctly in TATA-less promoters (help TFIID interact with SP1 that is bound to GC box) 2) TFIIA joins the complex (upstream of TFIID complex) --helps TFIID bind to TATA box How would you show this? 3) TFIIB joins the complex (downstream of TFIID/TFIIA complex) 4) TFIIF binds to RNA Polymerase II and helps it bind RAP74--associates with RAP30 and TFIIB RAP30--binds tightly to RNA Polymerase II Assembly of Basal Transcription Complex
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Assembly of Basal Transcription Complex 5)
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lecture17 - Factors Involved in Transcription Initiation...

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