[LAB1]_Lab1.5a. 10w.scope

[LAB1]_Lab1.5a. 10w.scope - Biology 05LA Winter Quarter...

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Biology 05LA – Winter Quarter 2010 Lab 1 – page 1 LAB 1 : INTRODUCTION TO LIGHT MICROSCOPY: THE COMPOUND MICROSCOPE: ITS CARE, COMPONENTS AND PRINCIPLES OF USE. Over the past 20 or so, the light microscope has become a most important tool in modern cell and molecular biology. In fact, a substantial part of the lecture content of Biology 5A that you will be hearing this quarter is derived from studies utilizing various forms of light microscopy. A feeling for the importance of light microscopy can be gained with a brief scan of your lecture text book (Biology, Campbell et. al., 8 th ed.). On page 113, the intracellular distribution of three classes of cytoskeletal elements is shown by the use of fluorescent tags. On page 7, the network of neurons that control the flight muscles of a birds is revealed by another light microscopic fluorescence technique. The localization of a specialized sequence of DNA on a chromosome is shown on page 319. And finally, the dynamics of the cytoskeleton as it participates in the process of mitosis is vividly demonstrated on pages 232 and 233. These few examples should convince you of the power of modern light microscopy. While you will not be using any of these techniques in Biol 05LA, it is very likely that you may later in your undergraduate studies or thereafter. It is the purpose of this laboratory to prepare you for this eventuality. The focus of this exercise will be in two areas. The first will be in the area of basic microscopy. Here you will be introduced to the parts of the microscope and how these parts interact to form an image. Then you will be introduced to some basic principles of light microscopy that are essential in all forms of microscopy. We will conclude by providing you with some basic guidelines for microscope use that have relevance to all forms of light microscopy. The second area of focus for this lab will be about how to handle the microscope in a manner that will not damage it. Microscopes are expensive and obtaining the best possible results in microscope studies requires that the instrument being used is in top working order. Please take heed of the safety guide lines that will be presented as you work through this exercise – your success in Biol 05LA and future studies will be enhanced if you do so. I. MICROSCOPE COMPONENTS Before getting started, you need to obtain a microscope. Your TA will describe the two-handed manner in which a micro- scope should be transported; one hand should firmly grasp the arm of the ± scope and the other should be palm up supporting the base . Once you have a microscope, take a few minutes to look Fig. 1 and familiarize Course focus Brightness control Illuminator Arm Diopter ring Base Fine focus Objective lens (1 of 4) Stage motion controls Condenser Mechanical stage assembly Mechanical stage assembly Condenser focus Binocular eyepiece tube Condenser diaphragm lever Oculars (10x) Revolving nosepiece Figure 1. Parts of the compound light microscope; The Nikon Alphaphot.
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[LAB1]_Lab1.5a. 10w.scope - Biology 05LA Winter Quarter...

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