Lab Quiz.preview

Lab Quiz.preview - filled tubes. (1.5 pts. total) a....

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
These are the questions that you will face on next week’s quiz. Seeing as you have the questions well in advance of the quiz, you will be expected to write complete, well-considered answers. NO NOTES WILL BE ALLOWED AT QUIZ TIME . 1. Spectrophotometer terminology. (1 pt. total) a. Define the terms absorbance (A) and transmittance (T) in terms of the amount of light entering (I o ) and leaving (I) a sample. b. Why is the log transform used in the expression of A? 2. The spectrophotometric blank. (1 pt. total) a. Explain why a blank is required for spectrophotometric studies. b. Describe how the blank for a particular study is chosen. 3. Relative to the expression for Beer’s law: (1 pt. total) a. How is the molar extinction coefficient ( E ) determined? b. What units are associated with E ? 4. You will be asked to do the calculations for a problem similar to the one you did in lab where you needed to determine the concentration of a K 3 Fe(CN) 6 solution (3 pts.) 5. Explain how variations in the following parameters influence the rate of diffusion in a simple system like our agar-
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: filled tubes. (1.5 pts. total) a. Increasing concentration of the test solute. b. Increasing the temperature of the tube and the solute. c. Increasing the molecular mass of the test solute. 6. Define the term osmolarity. (1 pt.) 7. Explain why osmolarity differences are more significant than molarity differences when trying to predict the direction of osmotic water movement. (1.5 pts.) 8. Explain why plant cells are able to live happily in a hypertonic environment while animal cells cannot. (1 pt.) 9. For the exercise where you estimated the osmolarity of some red blood cells, thoroughly explain why the lowest solute concentration at which the cells were unbroken represented a reasonable estimate of the osmolarity of the cells. (2 pts.) 10. Explain the difference between inductive and deductive reasoning. (1 pt.) 11. How does a hypothesis differ from a prediction. [hint – do not refer to a hypothesis as an educated guess.] (1 pt.)...
View Full Document

This note was uploaded on 04/06/2010 for the course BIOL 5LA taught by Professor Haimo during the Spring '08 term at UC Riverside.

Ask a homework question - tutors are online