Bio 101 Day 26

Bio 101 Day 26 - Recombinant DNA that have incorporated the...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Recombinant DNA that have incorporated the favorite gene cloning Gene Cloning in a plasmid --Human DNA fragments are cut with restriction enzymes (they have sticky ends now) --Fragments produce recombinant DNA --some Plasmids contain genes for resistance to antibiotics --E-Coli cells are put on petri-dish (plate) --Cloning of E-Coli cells (cellular) --Cloning of Recombinant DNA --Bacteria with plasmid live and multiply --Bacteria without plasmid fail to grow Genetic Probe --Radioactive DNA or RNA sequence used to screen recombinant DNA molecules in bacterial cells to find specific colony with DNA of interest “Molecular hybridization”—based on complementary base pairing --Petri-Dish contains colonies Transfer cells from colonies to nitrocellulose filter Filter with bacteria from colonies; cells are lysed and DNA denatures + Radioactively labeled nucleic acid probe is added some radioactive nucleic acid probe molecules become hybridized to DNA of some colonies Exposed X-Ray film; dark spots identify colonies with desired DNA --Use restriction enzyme to release desired fragment from Recombinant DNA Polymerase Chain Reaction (PCR) --Automated in vitro technique
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/06/2010 for the course BIO 01-119-10 taught by Professor D'arville during the Fall '09 term at Rutgers.

Page1 / 5

Bio 101 Day 26 - Recombinant DNA that have incorporated the...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online