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Chapter 9 - Chapter 1 a10 b1 c9 d7 e6 f2 g8 h3 i5 9 j4 3...

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Chapter 9 1. a10, b1, c9, d7, e6, f2, g8, h3, i5, j4 3. Selectable markers in vectors provide a means of determining which cells in the transformation mix take up the vector. These markers are often drug resistance genes, such that cells lacking the vector will not survive in medium with the drug. 6. The genomic library is based on the most inclusive and complex starting material so it would consist of the greatest number of different clones 12. A circular 9kb DNA molecule with a single Bam and a single Eco site separated by 3kb EcoRI BamHI EcoRI |----------------|--------------------------------| (if linearized) 3 kb 6kb 13. When the vector is digested with EcoRI you get one 2.4kb fragment. When the vector is digested with MboI there are three fragments 0.3, 0.5 and 1.6kb. The somatostatin insert was cloned into the vector at the EcoRI site. There is an EcoRI site very close to one end of the insert DNA. Therefore after digestion of the recombinant plasmid with EcoRI, a small EcoRI insert fragment of 49bp and the vector fragments of 900, 500, 300 and 700bp (2400 bp total length) will
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