Chapter 10 - diverged. 16) cDNAs lack regulatory region...

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Chapter 10 solutions: 5a) The man is homozygous for a mismatched sequence to which the Beta primers bind, preventing the sequence from being amplified. b) The man is heterozygous for the sequences to which the alpha and delta primers bind, because the sequence is amplified in about half of the sperm. There are 10 a+d+ sperm, 11 a-d- sperm, 2 a+d- sperm and 2 a-d+ sperm. The first 2 classes are parentals, so the man’s genotype is a+d+/a- d-. The last 2 classes are recombinants, so rf = 4/25 = 16 cM. Thus, the regions to which the alpha and delta primers anneal are 16 cM apart. 6a) Differences in the amount of recombination in males and females b) No, physical distances are the same. The same chromosomes are passed from generation to generation (i.e., grandmother to father to daughter) 14a) 2.73 kb b) 3.53 kb 15) cDNA clones are made by synthesizing a copy of the processed mRNA. The 2 different cDNA clones may be due to alternate splicing of the same primary RNA, or a gene duplication originally gave rise to 2 identical copies of a gene, the center portion of one of which has
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Unformatted text preview: diverged. 16) cDNAs lack regulatory region information and intron DNA 17) Pseudogenes lack introns, have a poly-A tail and do not have regulatory DNA sequences upstream of the gene. 18) i) Centromeres and telomeres are important for proper function of chromosomal structures. ii) Transposon-derived repeats have given rise to at least 47 different human genes. iii) Certain genes have repeated sequences which lead to mutation when these regions are amplified (i.e. Huntington’s disease). iv) Transposon-derived sequences have reshaped the genome by aiding the formation of chromosomal rearrangements 19a) The predicted amino acid sequence of the protein product can be examined for protein domains or functional units (protein motifs). b) Northern blot analysis to discover in when and in which tissue the gene is transcribed. Also, knocking out a gene and looking for a phenotype....
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This note was uploaded on 04/07/2010 for the course BIO 105 taught by Professor Sullivan during the Summer '08 term at University of California, Santa Cruz.

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