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Chapter 16 - 16-11(a When all the genes are wild-type there...

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Chapter 16 Solutions 16-1. a. 4; b. 8; c. 5; d. 2; e. 7; f. 1; 16-4. Mutations in the promoter region can only act in cis to structural genes immediately adjacent to this regulatory sequence. This promoter mutation will not affect the expression of a second, normal operon 16-5. a. lacZ is constitutive; lacY is constitutive b. lacZ is constitutive; lacY is inducible c. lacZ is inducible; lacY is inducible d. no expression of lacZ; lacY is constitutive e. no expression is lacZ; no expression of lacY 16-6. These revertants of the constitutive expression of the lac operon could be changes in the base sequence of the operator site that compensate for the mutation in lacI 16-7. see graph 16-9. statement (b) will be true
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Unformatted text preview: 16-11. (a) When all the genes are wild-type, there is no expression of the structural genes in the absence of arabinose and the structural genes are expressed in the presence of arabinose (b) The mutant version of araC blocks synthesis of the three structural gene products in the presence of arabinose 16-12. (a) the repressor protein (lacI) is bound (b) in the presence of glucose + lactose there will be no proteins bound to the regulatory region (c) the CAP-cAMP complex will bind to the promoter region 16-24 the wild type LexA protein negatively regulates the expression of these genes...
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