SolutionSet4 2009

SolutionSet4 2009 - BMB100B Winter 2009 Rubin Solution Set...

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BMB100B Winter 2009 Rubin Solution Set #4 Due Tuesday, February 10 th 10:00 A.M. 1) Derive the Michaelis-Menten equation for a mixed inhibitor (an inhibitor that binds both E and ES). You can assume that substrate binding to EI is negligible.
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BMB100B Winter 2009 Rubin 2) When proteins become phosphorylated, they often migrate differently in an electrophoresis gel. One can use this property to determine whether a protein substrate of a kinase has been converted into phosphorylated product; the phosphorylated product band will shift from the unphosphorylated substrate band on the gel. Propose a kinetic assay that could be used to measure the K I of the noncompetitive kinase inhibitor Gleevec. Some considerations: What are the known quantities in your enzyme catalyzed reaction? What is the data you actually measure and how do you measure it? How do you plot this data for analysis? Sketch examples. What kinetic constants do you extract from analysis of the data and how do you calculate them?
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BMB100B Winter 2009 Rubin How can you vary the reaction conditions (i.e. known quantities in the reaction) to manipulate the kinetic constants and derive K I?
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BMB100B Winter 2009 Rubin
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BMB100B Winter 2009 Rubin 3) Read the recent review article by James Robertson entitled “Mechanistic Basis of Enzyme- Targeted Drugs” in the journal Biochemistry. The reference is as follows: Robertson, J.G. Biochemistry, Volume 44, Issue 15, pages 5561-5571, 2005.
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SolutionSet4 2009 - BMB100B Winter 2009 Rubin Solution Set...

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