Unformatted text preview: Stickase Lab Activity" In this la w rk to better understand principles of Michaelis- Menten enzyme kinetics. A
student will act as an enzyme by grabbing a toothpick from the bag (substrate) into his/her hand (active
site), breaking it in half and putting the half pieces into another bag (product). The data you collect
will be used to determine different kinetic parameters of this reaction. Group Size: 2—3 People Materials Per Group: 2 Paper Bags labeled “Product” and “Substrate”, 1 box of toothpicks, and 20
colored toothpicks Directions: 1) Assign jobs: 1 of you will be the enzyme for the entire activity, and the other(s) will time and
record data 2) Initial rates (V0) are useful measures of catalytic activity. You will measure the V0 by counting
the toothpicks broken in the course of 30 seconds at varying substrate concentrations, [S]. [S] (toothpicks in substrate bag) V0 (toothpicks broken/30 seconds)
100 3) Graph your data: (091481“ l'lhf:
(Latent faﬁlnrfh V0
(# toothpicks Will ‘ -«. 7'"?- .__
broken! (1 4M“; ) ﬂ; .
30 seconds) M:
K U""T"\ M f NJ (r (More) 0:? «I [S] (# toothpicks) o a c we} VC .5 rec C 6 5”th\ cl; Mikel wlrrt‘ﬂ “L: d: L. B 4) Label the graph showing Illegal“ and Km. Estimate these and give proper units: UL“ clad " 7 C "9‘ Vmax = K111 : * Adapted from: Runge, Steven W.= Hill, Brent J.F., Moran, William M. A Simple Classroom Teaching Technique To Help
Students Understand Michaelis—Menten Kinetics. CBE Life Sci Educ 2006 S: 348-352 ...
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- Winter '10