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Stickase Lab Activity Key 003

Stickase Lab Activity Key 003 - 14 To imitate the effect of...

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Unformatted text preview: 14) To imitate the effect of one type of inhibitor, add 10 colored toothpicks to your substrate bag. As you do the next set of V0 measurements with [I] = 10 (# inhibitors), if you happen to pick up a colored toothpick, you must put it back into the substrate bag; it cannot be broken into product. [S] (+ [I] = 10 colored toothpicks) V0 (toothpicks broken/30 seconds) 5 10 25 50 100 15) Graph this data on the previous graph from question #3 (label your data appropriately) 16) Label the graph showing the effective VW and Km. Estimate these and give proper units: Effective Vmax = Effective Km: 17) How did these parameters change with respect to the original graph without inhibitor added? \f M Y ~— W 18) What type of inhibitor is the colored toothpick? How do you know? . “WV”; , (Mai? “‘i Ly] { me JFXLP'yf “WC V. W‘% 1466' C5,; Hi?) A}; “l -c\ i as 1 MW" - ( 0pr 19) Write the Michaelis—Menten erjiation for this type of inhibitor: hr [E][5 7 ____ V0: (Him—v [5‘3 33$: \ ii L1 E Car 20) When [S] << Km the Michaelis-Menten equation simplifies to V0 : [ET] [iii 21) When [S] >> Km the Michaelis-Menten equation simplifies to V0 = VH1“: @ tech [ET] 22) Given these equations, derive the effective kcat of your enzyme (assume [E] :‘ljfi enzymes): F“"“~--\\W/J Z \C’CCX (J {3 Effective kcat = ...
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