Exp_5_Key - MCB 120L Winter 2009 EXPERIMENT 5...

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MCB 120L, Winter 2009 KEY Experiments 5 Morand EXPERIMENT 5 Representative data SDS-PAGE 1. photo attached 2. Table: Molecular Weights as determined by SDS-PAGE Protein MW (Dal) log MW Rm on gel bovine serum albumin 66,000 4.82 ~ 0.23 ovalbumin 45,000 4.65 ~ 0.37 carbonic anhydrase 29,000 4.46 ~ 0.55 β -lactoglobulin 18,400 4.26 ~ 0.80 lysozyme 14,300 4.16 ~ 0.91 Bovine Muscle LDH 32,000 to 36,000 4.50 to 4.60 ~ 0.48 Bovine Heart LDH 32,000 to 36,000 4.50 to 4.60 ~ 0.46 Porcine Heart LDH 32,000 to 36,000 4.50 to 4.60 ~0.46 3. Standard curve SDS-PAGE Standard Curve y = -1.0451x + 5.2434 R 2 = 0.9936 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Log MW (log kD) Rm 1
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MCB 120L, Winter 2009 KEY Experiments 5 Morand 4. Reported in table in question 2 5. Number of subunits per holoenzyme (example values) (140,000 Dal/holoenzyme) / (33,500 Dal/subunit) = 4.18 subunits/holoenzyme ~ 4.0 subunits/holoenzyme the number of subunits must be reported as a whole number (the active enzyme does not have a partial subunit) Native-PAGE 1. photo is attached 2. Table: Rm’s of the LDH isozymes Source Isozyme Rm on gel Bovine LDH (from muscle) M4 ~ 0.078 Bovine LDH M3H1 ~ 0.17 Bovine LDH M2H2 ~ 0.25 Bovine LDH
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Exp_5_Key - MCB 120L Winter 2009 EXPERIMENT 5...

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