RNAi practice problems-1

RNAi practice problems-1 - MCB121 W09 RNAi practice...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
MCB121 W09 RNAi practice problems S. Burgess 1. Name the three required components of RNAi and their functions. 2. Both siRNA and miRNA can silence gene expression at the post-transcriptional level, however the mechanisms differ. In a general way, how do the two processes differ? 3. What key difference between the structure of siRNA and miRNAs directs them to one or the other PTGS mechanisms? ( excluding plants) 4. What is the source of double-stranded RNA that leads to the formation of heterochromatin at centromeres in S. pombe. 5. Virtually no RNA products transcribed from the cen locus in S. pombe can be detected by rtPCR. By contrast, high levels of product were observed in dcr1- (dicer) mutants. Describe two mechanisms that are perturbed in dcr1- mutants that would lead to this phenotype. 6. RNAi an important genetic tool for genetic research in C. elegans . Interestingly only a small amount of RNA is required to elicit gene silencing throughout the animal and its progeny. It is often used to infer something about wild type gene function. A. What form is the RNA generally introduced into the animal?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/07/2010 for the course MCB 102,103,12 taught by Professor Segel during the Spring '10 term at UC Davis.

Page1 / 3

RNAi practice problems-1 - MCB121 W09 RNAi practice...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online