{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

MGT 3121 Spring 2014 Midterm Examination

MGT 3121 Spring 2014 Midterm Examination - Name Last First...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Name MCB121, Page 1 of 13 Last, First Burgess, Gasser MCB121-01, Winter, 2008 Final Exam, March 19, 2008 Instructions: There are thirteen pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. Amino acid Abbreviations Amino acid Abbreviations Alanine Ala A Leucine Leu L Arginine Arg R Lysine Lys K Asparagine Asn N Methionine Met M Aspartic acid Asp D Phenylalanine Phe F Cysteine Cys C Proline Pro P Glutamine Gln Q Serine Ser S Glutamic Acid Glu E Threonine Thr T Glycine Gly G Tryptophan Trp W Histidine His H Tyrosine Tyr Y Isoleucine Ile I Valine Val V Question Value Score 1 16 2 20 3 27 4 12 5 18 6 12 7 12 8 10 9 18 10 12 11 19 12 14 13 10 Total 200 Note: W09 we covered alternative splicing and DSB repair after MTII. So, expect the W09 final will be weighted to assign more points to those topics.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Name MCB121, Page 2 of 13 Last, First Burgess, Gasser 1. (16) Sic1 is a protein that is involved in regulation of progression through the cell cycle in yeast. The rate of transcription of the Sic1 gene does not vary during the cell cycle, but the level of Sic1 protein drops dramatically at the start of the G1 stage of the cell cycle. Sic1 is a target for phosphorylation by a protein called G1 cyclin. The level of this G1 cyclin varies during the cell cycle. Phosphorylated Sic1 is a target for ubiquitation by the E3 ubiquitin ligase, SCF CDC4 . Ubiquitination targets Sic1 for degredation by the proteasome. Assuming that the following changes do not affect yeast viability, what effect would each of the following be likely to have on the level of Sic1 in the cell at the start of the G1 stage of the cell cycle. Circle “I” for increase in level, “D” for decrease in level or “N” for no alteration from the wild-type levels. I D N A mutation that leads to overexpression of the G1 cyclin. (more phosphorylation of Sic1 = more degradation) I D N A mutation in SCF CDC4 that makes it able to add only a single ubiquitin to each Sic1 protein. (monoubiquitinated proteins are not targeted to the proteasome) I D N A mutation replacing the lysine residue in Sic1 to which ubiquitin is normally attached, with an alanine residue. (cannot attach ubiquitin to alanine) I D N Addition of the compound MG-132, a potent inhibitor of the protease activity of the proteasome. (would inhibit degradation of Sic1) 2. (20) For each of the following, put an “X” in the blank next to the one correct answer to each question. a) (5) Which of the following would be the best source from which one could isolate a DNA fragment that would include the complete promoter region of the mouse c-Myc gene?
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern