MC121FinalIvoryKey

MC121FinalIvoryKey - Name Last First MCB121 Page 1 of 13...

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Name MCB121, Page 1 of 13 Last, First Gasser, Burgess MCB121 Final Exam, Mar. 19, 2009 Instructions: There are thirteen pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. Amino acid Abbreviations Amino acid Abbreviations Alanine Ala A Leucine Leu L Arginine Arg R Lysine Lys K Asparagine Asn N Methionine Met M Aspartic acid Asp D Phenylalanine Phe F Cysteine Cys C Proline Pro P Glutamine Gln Q Serine Ser S Glutamic Acid Glu E Threonine Thr T Glycine Gly G Tryptophan Trp W Histidine His H Tyrosine Tyr Y Isoleucine Ile I Valine Val V Question Value Score 1 17 2 9 3 20 4 8 5 10 6 16 7 8 8 8 9 27 10 20 11 10 12 15 13 16 14 16 Total 200
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Name MCB121, Page 2 of 13 Last, First Gasser, Burgess 1. (17 points ) You have developed a computer algorithm to identify RNA secondary structure within intron sequences in mammalian cells. You find a sequence you predict would fold into a pre-miRNA in a gene called IPASSED . There is a gene in the genome called MISERY that contains complementary sequences in the 3’ untranslated region. A. (8) This pre-miRNA would likely be processed to miRNA by which enzymes? (circle all that apply) 1. Dicer 2. Drosha 3. Slicer 4. RNA dependent RNA polymerase Note: The question explicitly asks for the enzyme that processes the pre-miRNA, however, full credit will be given to those who chose Drosha as well since the distinction between pri-miRNA and pre-mRNA was not emphasized on the practice final last year B. (4) Would you expect the miRNA found in IPASSED to activate or repress MISERY expression? (circle one) C. (5) What is the likely fate of MISERY in a cell where IPASSED is expressed? (circle one) i. Chromatin surrounding the MISERY gene will be highly methylated? ii. The MISERY transcript will be subject to cleavage by slicer. iii. Translation will be blocked and the message will eventually degrade Note: since the miRNA comes from an animal cell, the miRNA does not slice the mRNA as it does in plants. iv. The MISERY protein can now bind to a histone deacetylase.
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Name MCB121, Page 3 of 13 Last, First Gasser, Burgess 2. ( 9 points ) In Drosophila, Sex lethal protein (Slx) is required for the female-specific splicing of tra mRNA (shown below). A. (5) Slx binds to the unusual poly-pyrimidine tract preceding exon 2 and excludes binding of U2AF. Like U2AF, Sxl protein contains an RNA binding domain but lacks an activating SR domain found in U2AF. You have created transgenic flies that make a fusion protein containing the RNA binding domain of Sxl and the SR activating domain of U2AF. From the choices below select the answer that best describes the interaction of the SXL-SR fusion.
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