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RelativeResourceManager(16) - Capillary electrophoresis and...

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Capillary electrophoresis and how it saved the Human Genome Project Chem 420 October 29, 2008 Professor Ryan C. Bailey
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Electrophoresis z Charged molecules migrate in response to an applied electric field z Direction of movement depends upon sign of charge. Opposites attract. z Rate of migration depends on magnitude of field, charge, and frictional retarding factors 2 e cm is the electrophoretic mobility ( ) Vs V E is the applied field strength ( ) cm e vE μ =
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Electrophoretic separations z Species that differ in charge or frictional forces will migrate at different rates z Neutrals don’t separate z If ions are same size, then species with more charge will migrate faster. z If the species have the same charge, then they will migrate according to size z smaller = faster z Charge-to-size ratio
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Electrophoretic separation formats z Slab-based electrophoresis z Poly(acrylamide) or agarose gels z Slow, laborious, hard to automate and just generally sucks z Capillary electrophoresis z Developed in the mid- to late 1980s z Electrophoresis performed in silica (glass) capillaries with high speed, high resolution, and on very small volumes (sub μ L) z Samples are easily collected for further analysis because they elute from the capillary http://en.wikipedia.org/wiki/Gel_electrophoresis http://colbychiang.com/blog/wp-content/uploads_raeo89nd/2008/02/gel_electrophoresis.gif
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Capillary electrophoresis V is the applied voltage L is the length of the column ee V vE L μμ ==
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Resolution in CE z Single phase means no mass transfer band broadening.
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RelativeResourceManager(16) - Capillary electrophoresis and...

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