Lecture 12 - HMB265 Human General Genetics Lecture 12...

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Lecture 12: Genetic Dissection of Complex Traits Prof. Maria Papaconstantinou
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Lecture Outline Mapping Complex Traits in Plants Mapping Complex Traits in Humans From linkage map locus to candidate gene Haplotype Analysis Association Studies Reading: Griffiths et al ., 9 th edition, Chapters 4 & 18 ( emphasis on pages 146-153,158-159, 163-164, 664-668 )
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Dissecting the basis of tomato fruit size STEP 1: Make an informative cross
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STEP 2: Determine the frequency distribution in the F 2
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Most loci will show this type of segregation pattern STEP 3: Use molecular markers to “genotype” the individuals, attempting to find markers that co-segregate with the trait
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STEP 3: Use molecular markers to “genotype” the individuals, attempting to find markers that co-segregate with the trait
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STEP 4: Use a statistical method to determine if markers are co-segregating (associating) with the trait or not
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Most loci will show this type of segregation pattern STEP 4: Use a statistical method to determine if markers are co-segregating (associating) with the trait or not
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STEP 5: Plot the degree of association (logarithm of odds - LOD score) on a linkage map
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The power of genetics… Dissecting the basis of tomato fruit size Lycopersicon esculentum cv. Giant Heirloom 1 kg Lycopersicon pimpenellifolium 1 g Anne Frary et al . (2000) Science 289: 85 -88
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The power of genetics… Dissecting the basis of tomato fruit size Step 1 : QTL controlling tomato fruit size were mapped Anne Frary et al . (2000) Science 289: 85 -88
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The power of genetics… Dissecting the basis of tomato fruit size Step 1 : QTL controlling tomato fruit size were mapped Anne Frary et al . (2000) Science 289: 85 -88
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The power of genetics… Dissecting the basis of tomato fruit size Step 2 : Create recombinant inbred lines (RILs) Also known as near-isogenic lines (NILs) Generated by inbreeding selected individuals, down a lineage Each generation 1-2 siblings within each lineage are inbred,
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This note was uploaded on 04/08/2010 for the course HMB 265 taught by Professor French during the Spring '09 term at University of Toronto.

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Lecture 12 - HMB265 Human General Genetics Lecture 12...

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