Lab Exam Notes - Lab Exam Notes Lab 1 Prelab Microscopes on...

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Lab Exam Notes: Lab 1: Prelab: - Microscopes on the end of the bench - Carry by the handle - Coloured sticker faces up - 4x = 1.75 mm - 10x = 0.70 mm - 40x = 0.175 mm - 100x = 0.07 mm Internet: Know your microscope, functions and parts Lab Manual: - Parfocal = Some element of focus will remain when changing objectives - Human eye resolving power: 100 microns - 10000 microns = 10 mm = 1cm - The actual object turns upside-down/around when in the compound microscope - Working distance decreases with increased magnification - Threads went: Yellow, blue, red - Stereoscope: 3D image that is NOT inverted. - Brine Shrimp Larvae (Artemia) - Protoplast = protoplasm = cytoplasm/fluid of the cell - Lyngbya: Appears green, no nucleus, moving (not observed) filaments - Epiphytic Diatom: Brown plant that grows on another (Elodea in this case) - Elodea: Leaf specimen used - Chlorophyll is responsible for green colouring - Photosynthesis is the important biological process of chloroplasts (discs) - Cytoplasmic Streaming (Cyclosis): moves chloroplasts to an area of better sun exposure. - Plasmolysis: Shrinking of the protoplast due to osmosis of water out of the cell - The stuff between the cell wall and the plasma membrane is the same as what is outside the cell. Lab 2: Prelab: - Record times every 30 seconds - If hemolysis (red blood cell explosion) occurs right away, record it as <2 Internet: - Electrolyte: A solute that dissociates into ions when put into a solution. - Diffusion: Movement of a solution from one concentration (higher) to another (lower). - Osmosis: Movement of water across a semi-permeable membrane (higher to lower concentration) - Osmolarity: Concentration of all solutes in a solution.
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- Tonicity: Globally, tonicity depicts the ability of a solution to cause a cell to absorb or reject water. It depends on cell membrane permeability, as well as concentration. - Hypotonic: A solution that causes water to enter the cell. For non-permeating: concentration is lower outside the cell. Lab Manual: - Erythrocytes: Large number of homogenous cell, that keep for a long time. - Hemolysis: Rupture of the red blood cells - Pigment = Hemoglobin - Hypo = Water enters - Isosmotic = same osmotic pressure Lab Results: - Occurred Fast: water, Urea, Triton X-100, Ethanol, Ethylene Glycol - Medium speed: Thiourea - Slow: Sucrose, Dextrose, Glycerol Lab 3: Internet: Amoeba Structures: 1. Pseudopodium: “finger projections” 2. Uroid (Region): Tail region, contracting side 3. Cell Membrane/Plasmalemma: Outer surrounding of the cell 4. Ectoplasm: Viscous clear outer layer (no motion) 5. Endoplasm (granular): Granular fluid layer (motion) 6. Hyaline Cap: Tip of the “reaching” side of the amoeba 7. Nucleus: The clearly defined dark staining portion 8. Contractile Vacuole: Spherical, collects the fluid and expels it 9. Digestive/Food Vacuoles: Spherical, contains contents to be digested 10. Triuret Crystals: Rectangular in shape. Extras:
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This note was uploaded on 04/08/2010 for the course MATH MAT1330 taught by Professor Rad during the Spring '10 term at University of Ottawa.

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Lab Exam Notes - Lab Exam Notes Lab 1 Prelab Microscopes on...

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