{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}


BIOLOGY FINAL PRACTICE EXAM - 51-53 Complementary base...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
51-53 . Complementary base pairing is a fundamental property of nucleic acids. How is base pairing involved in each of the following procedures? 51. Making recombinant DNA a. Sticking plasmids into bacteria. b. Cutting a plasmid open with a restriction enzyme. c. Ligating a cDNA into the plasmid by hybridization. d. Inserting a cDNA into a plasmid using sticky ends. e. Transcribing DNA to make an RNA probe. 52. In situ hybridization 53. Microarrays 54. Why is reverse transcriptase useful in gene cloning? 55. Which sequence is most likely to function as a restriction enzyme site? (Remember that DNA is double stranded.) a. CTAGGG b. AAATTTCCC c. GGATCC d. GATTAG c. ATCATC 56. DNA sequencing (Sanger method) is based on: 57. In DNA sequencing (Sanger method), ddATP:
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
58. Why would Sanger DNA sequencing fail if there were 10 times more of each ddNTP relative to each dNTP?
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}