BIOLOGY FINAL PRACTICE EXAM - 51-53. Complementary base...

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51-53 . Complementary base pairing is a fundamental property of nucleic acids. How is base pairing involved in each of the following procedures? 51. Making recombinant DNA a. Sticking plasmids into bacteria. b. Cutting a plasmid open with a restriction enzyme. c. Ligating a cDNA into the plasmid by hybridization. d. Inserting a cDNA into a plasmid using sticky ends. e. Transcribing DNA to make an RNA probe. 52. In situ hybridization a. Showing identity between sense (S) RNA and mRNA. b. Bonding between antisense (AS) RNA and the complementary DNA strand. c. Recognizing an mRNA with an antisense (AS) RNA probe. d. Bonding between two anti-parallel DNA strands. e. Binding a specific antibody to the mRNA. 53. Microarrays a. Reacting exon DNA to glass slides. b. Providing cDNAs with fluorescent labels. c. Comparing exons and introns attached to slides. d. Binding labeled cDNAs to exons. e. Making labeled exons for identification of RNAs. 54. Why is reverse transcriptase useful in gene cloning? a. It provides sites for restriction enzymes. b. It promotes entry of plasmids into bacterial cells. c. It makes RNA copies of genes. d. It allows retroviruses to infect cells. e. It makes DNA copies of mRNAs. 55. Which sequence is most likely to function as a restriction enzyme site? (Remember that DNA is double stranded.) a. CTAGGG b. AAATTTCCC c. GGATCC d. GATTAG c. ATCATC 56. DNA sequencing (Sanger method) is based on: a. transcribing the DNA into RNA and then to cDNA. b. using the genetic code on protein sequences. c. placing different labels on the bases in a strand of DNA. d. producing different sized fragments by DNA synthesis. e. cutting DNA with many different restriction enzymes. 57. In DNA sequencing (Sanger method), ddATP: a. serves as the energy source for DNA synthesis. b. is inserted instead of dTTP and causes a mutation. c. base pairs with C instead of T and bends the DNA. d. is added to the 5’ end of short DNA fragments. e. adds at the 3’ end instead of dATP and stops DNA synthesis.
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58. Why would Sanger DNA sequencing fail if there were 10 times more of each ddNTP relative to each dNTP? a. Double stranded DNA would not form. b. The whole DNA strand would be copied. c. Only a few short DNA fragments would be produced. d. The wrong bases would be added to DNA fragments. e. Statement is false; sequencing would be successful. 59. The following can be used as vectors for cloning and manipulating genes: a. SCID, plasmid, chromosome. b. primer, plasmid, peroxisome. c. retrovirus, Ti plasmid, BAC. d. Ti plasmid, MT promoter, GM plant e. Bt toxin, adenovirus, plasmid. 60. Introduction of a gene to correct a human disease is being attempted on somatic cells but not on germ cells because: a. it is easier to get genes into somatic cells. b. foreign genes are stable in somatic cells but not in germ cells.
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BIOLOGY FINAL PRACTICE EXAM - 51-53. Complementary base...

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