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BIOLOGY FINAL PRACTICE EXAM

BIOLOGY FINAL PRACTICE EXAM - 51-53 Complementary base...

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51-53 . Complementary base pairing is a fundamental property of nucleic acids. How is base pairing involved in each of the following procedures? 51. Making recombinant DNA a. Sticking plasmids into bacteria. b. Cutting a plasmid open with a restriction enzyme. c. Ligating a cDNA into the plasmid by hybridization. d. Inserting a cDNA into a plasmid using sticky ends. e. Transcribing DNA to make an RNA probe. 52. In situ hybridization 53. Microarrays 54. Why is reverse transcriptase useful in gene cloning? 55. Which sequence is most likely to function as a restriction enzyme site? (Remember that DNA is double stranded.) a. CTAGGG b. AAATTTCCC c. GGATCC d. GATTAG c. ATCATC 56. DNA sequencing (Sanger method) is based on: 57. In DNA sequencing (Sanger method), ddATP:
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58. Why would Sanger DNA sequencing fail if there were 10 times more of each ddNTP relative to each dNTP?
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