Lecture17.2010 - Cell-Cell Adhesion and Cell Junctions...

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Cell-Cell Adhesion and Cell Junctions Lecture 17 3/25/10 Reading: Alberts Chapter 19, pgs. 1131-1163 . Cell-cell interactions and Connections Interactions of Cells with Other Cells I. Little known about mechanisms that generate complex 3-D cell arrangements in developing organs -presume to depend heavily on selective interactions between cells of II. Experiments demonstrating cell adhesion A. Remove developing organ from chick or amphibian embryo, dissociate to single cells 1. Disaggregate cells from 2 different developing organs, mix - form mixed clump then sort out 2. Once separated into homogeneous cluster, cells often differentiate into structures they would have formed within intact embryo B. Add labeled cells to dishes with monolayers of varied cell types -> cells stick to own type better III. Four distinct families of membrane proteins are most important in mediating cell-cell adhesion A. Selectins B. Certain members of immunoglobulin superfamily (IgSF) C. Certain members of integrin family D. Cadherins IV. Selectins - integral glycoprotein family; binds to specific carbohydrate groups in oligosaccharides on cell surface; name comes from lectins, compounds that bind specific carbohydrate groups A. Has small cytoplasmic domain, a membrane-spanning domain, & a large extracellular segment that consists of a number of separate domains (outermost domain acts as lectin) B. 3 known types - E-selectin (endothelial cells); P-selectin (platelets, endothelial cells); L-selectin (present on all types of leukocytes or white blood cells). C. All recognize similar grouping of 4 sugars present on ends of certain carbohydrate chains on glycoproteins. All require calcium for binding to their carbohydrate ligands. D. Mediate transient interactions between circulating leukocytes & vessel walls at V. Immunoglobulin Superfamily - large family of proteins with similar domains; most present on lymphocyte surfaces as integral proteins A. Most IgSF members involved in various aspects of immune function but some mediate
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This note was uploaded on 04/10/2010 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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Lecture17.2010 - Cell-Cell Adhesion and Cell Junctions...

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