Genetically Modified Organisms 2009 Lecture

Genetically Modified Organisms 2009 Lecture - Genetically...

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Purpose of Lab To use PCR and gel electrophoresis to test for the presence of genetically modified plant products often found in common foods.
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Four Lab Objectives for our GMO Experiment Week 1 : 1. Extract DNA from food samples 2. Set up PCR reactions Week 2 : 3. Electrophoresis of PCR Products 4. Analysis of gel results
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How? About 85% of all GM Foods currently available can be detected with our reagents.
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Beauty of PCR: What is Needed? 1. Minute quantities of the DNA template 2. DNA polymerase 3. Two DNA primers 4. DNA base units 5. Buffers and cofactors http://images.the- scientist.com/content/figures/0890-3670- 040927-44-1-1.jpg
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What does an engineered gene need?
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35S promoter from the cauliflower mosaic virus Promoter: Docking site for RNA polymerase Signal for where transcription should begin. 35s promoter is chosen b/c it is already designed by nature to activate transcription in all plant cell types Activate transcription in all plant cell types
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We Also Need a Terminator Terminator of the Nopaline synthase (NOS) gene from Agrobacterium tumefaciens https://eapbiofield.wikispaces.com/file/vi ew/c7.17.7b.transcription.jpg
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Test for the presence of two different GMO-associated sequences: 35S promoter of the cauliflower mosaic virus (CaMV 35S). Terminator of the nopaline synthase (NOS)
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Genetically Modified Organisms 2009 Lecture - Genetically...

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