Assignment 2 - AMATH/BIOL 382 Problem Set 2 Due February 3...

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Unformatted text preview: AMATH/BIOL 382 Problem Set 2 Due February 3, 2010 1. Recall the derivation of the Michaelis-Menten description of an enzyme catalysed reaction (refer to Figure 1). If the back reaction C ← P + E is not assumed negligibly slow, the k 1 k-1 k 2 k-2 C E + P S + E . Figure 1: enzyme-catalysed reaction analysis needs to be extended. a) Write down a complete description of the system dynamics ( not assuming k- 2 = 0). b) Making the quasi-steady state assumption, show that c = k 1 e s + k- 2 e p k 1 s + k- 2 p + k- 1 + k 2 c) Determine the overall reaction rate (i.e. the net rate of production of product) as a function of the concentrations of substrate and product. d) Confirm that when k- 2 is taken to be zero, the standard form for Michaelis-Menten kinetics is recovered. 2. a) How would you estimate the Michaelis-Menten parameters V max and K m from a Lineweaver-Burke plot : a linear plot of 1 /V against 1 /s (where V is the rection rate, or velocity)....
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This note was uploaded on 04/11/2010 for the course CHEM 1101 taught by Professor Leroy during the Spring '10 term at University of Toronto.

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Assignment 2 - AMATH/BIOL 382 Problem Set 2 Due February 3...

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