Test 2 - 1. How would you prepare 750 mL of a solution...

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1. How would you prepare 750 mL of a solution consisting of 50 mM NaCl, 100 mM Tris, 15 mM EDTA, and 20 μ g/mL RNaseA? You have available to you stock solutions of 5 M NaCl, 2 M Tris, 1 M EDTA, and 50 μ g/ μ L RNaseA. Express your answers in the simplest form with the most logical units, and show all work for full credit. 2. You have run out of some DNA that you often use, so you go to the freezer to get your stock tube of nice, clean maxiprep DNA. Unfortunately, some of your label has rubbed off, and you can’t read the concentration. You remember that the concentration was relatively high, so you dilute 10 μ L of this DNA with 90 μ L of water, and then you take 5 μ L of this into 995 μ L of water in a quartz cuvet, and read the absorbance of this solution at 260 nm. You read an absorbance of 0.12. You remember that a 50 μ g/mL solution of double-stranded DNA will give an absorbance of 1.0 at 260 nm. What is the concentration of the DNA in your stock tube? Show all calculations for full credit. 3.
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This note was uploaded on 04/11/2010 for the course BICD bicd 100 taught by Professor Soowal during the Winter '08 term at UCSD.

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Test 2 - 1. How would you prepare 750 mL of a solution...

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