Unformatted text preview: d. How was this ELISA reaction set up? Draw a diagram of a well and the components in it. (There are several possibilities – try to keep it simple!) 1. Western blotting is the electrophoretic transfer of proteins from a gel to a filter. a. How do we set up the electrodes for the transfer with respect to the gel and the membrane? (Diagram is probably the easiest here.) b. What type of forces bind the protein to the membrane? c. What ingredient in the transfer buffer helps to ensure that these forces (from part b) are as strong as possible? How does this work? 2. Pregnancy tests detect the hormone hCG (human chorionic gonadotropin), which is produced by the embryo. On the following templates (not shown, but it is just a basic test) , label the reagents present at each zone, draw a simple diagram showing the antibodies and antigens (include labels!), and draw where you would expect to see color in the positive and negative tests....
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- Winter '08
- Monoclonal Antibodies, Human chorionic gonadotropin, estradiol, ELISA reaction