BIO320_HW1_2010 - Name BIO320 HW-1(20 points Due Tuesday...

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Name _____________________________________ BIO320 HW-1 (20 points) Due Tuesday February 2 nd , 2010 Please turn in your printed copy to class Please write legibly, preferably by computer Problem 1) The resolving power of a light microscope depends on the width of the cone of light that illuminates the specimen, the wavelength of the light used, and the refractive index of the medium separating the specimen from the objective and condenser lenses, according to the formula: resolution = 0.61 λ n sin θ where λ equals the wavelength of light used, n is the refractive index, and θ is half the angular width of the cone of rays collected by the objective lens. Assuming an angular width of 120° ( θ = 60°), calculate the various resolutions you would expect if the sample were illuminated with violet light ( λ = 0.4 µm) or red light ( λ = 0.7 µm) in a refractive medium of air (n = 1.00) or oil (n = 1.51). Which of these conditions would give you the best resolution? Problem 2) You hate the smell of mercaptoethanol. Since there are no disulfide bonds in intracellular proteins, you have convinced yourself that it is not necessary to treat a cytoplasmic homogenate with mercaptoethanol prior to SDS-PAGE.
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