BIO320_HW1_2010

# BIO320_HW1_2010 - Name BIO320 HW-1(20 points Due Tuesday...

This preview shows pages 1–2. Sign up to view the full content.

Name _____________________________________ BIO320 HW-1 (20 points) Due Tuesday February 2 nd , 2010 Please turn in your printed copy to class Please write legibly, preferably by computer Problem 1) The resolving power of a light microscope depends on the width of the cone of light that illuminates the specimen, the wavelength of the light used, and the refractive index of the medium separating the specimen from the objective and condenser lenses, according to the formula: resolution = 0.61 λ n sin θ where λ equals the wavelength of light used, n is the refractive index, and θ is half the angular width of the cone of rays collected by the objective lens. Assuming an angular width of 120° ( θ = 60°), calculate the various resolutions you would expect if the sample were illuminated with violet light ( λ = 0.4 µm) or red light ( λ = 0.7 µm) in a refractive medium of air (n = 1.00) or oil (n = 1.51). Which of these conditions would give you the best resolution? Problem 2) You hate the smell of mercaptoethanol. Since there are no disulfide bonds in intracellular proteins, you have convinced yourself that it is not necessary to treat a cytoplasmic homogenate with mercaptoethanol prior to SDS-PAGE.

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

### What students are saying

• As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

Kiran Temple University Fox School of Business ‘17, Course Hero Intern

• I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

Dana University of Pennsylvania ‘17, Course Hero Intern

• The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

Jill Tulane University ‘16, Course Hero Intern