DQ3-1 - that needs to have both the N-terminus and...

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Discussion question set 3 Chapter 12: Intracellular Compartments and Protein Sorting Overview of Secretory Pathway 1) Cytosolic proteins are translated by ribosomes 2) What are the functions of SRP during translation or a peptide across the ER membrane? 3) For a secreted protein, how will the migration pattern on a SDS gel differ if the protein was translated via in vitro translation system (free of ER) vs. endogenously by the cell? Why? 4) Diagram or describe two (2) ways a single pass transmembrane protein can have its N- terminus in the ER lumen.
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5) What type of signal sequences would be required to insert a protein into the ER lumen
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Unformatted text preview: that needs to have both the N-terminus and C-terminus in the cytosol for proper function? Chapter 13: Intracellular Vesicular Traffic Coated Vesicles, Rabs and Snares 1) What are two (2) functions of protein coats? 2) What family of protein is responsible for Coat protein assembly and disassembly? 3) Please label where vesicle coats proteins along with their accessory proteins function during membrane trafficking (@ ER, Golgi, and Plasma membrane). 4) How does Sar1 influence the formation of vesicles that bud from the ER membrane? What other proteins is required for the activity of Sar1?...
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DQ3-1 - that needs to have both the N-terminus and...

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