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DQ4 - probability of the t-snare and v-snare will have a...

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Discussion question set 4 Chapter 13: Overview of Membrane Trafficking I. SNAREs and Rabs ( page 749-765 ) 1a) What is the cellular localization of Rab, Rab effector proteins, and SNAREs for vesicle trafficking. b) Describe how Rab and its effector proteins work with SNAREs to provide specificity for vesicle trafficking. Rab proteins are small GTPases. Rab effector proteins work with the Rab proteins. Rab proteins are on the vesicle. The corresponding effector protein is on the target vesicle. When you allow interaction between the snares, you will allow for fusion. Rab proteins are not required for fusion. The Snares are required for fusion…….. c) How would the experimental removal of v-SNAREs or t-SNARES from a cell affect membrane vesicles? If you remove the snares, there will be no fusion. You won’t replenish vesicle membranes? ………….Botox breaks the T-snares…
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d) How would the experimental removal of Rab or its effector proteins from a cell affect membrane vesicles? If you remove Rab or its effector proteins, the rate of fusion would decrease substantially, since the
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Unformatted text preview: probability of the t-snare and v-snare will have a harder time finding eachother without the Rab. Fusion will still occur but it will be much slower. II. ER to Golgi (page 766-778) 1a) The diagram below is the primary confirmation for a hypothetical protein. Using an antibody to the protein, where would you expect it to be localized? Describe how the localization of the protein will change if we replace different portions of the protein with GFP. b) Substitution of ERSS with GFP: N--C ERSS KDEL Signal peptidase Cleavage site N--C GFP KDEL Signal peptidase Cleavage site c) Substitution of internal portion with GFP: d) Substitution of KDEL with GFP: e) For each of the proteins diagramed above (a-d), how will the protein distribution change if we neutralize the pH level in the Golgi (more basic now)? N--C ERSS KDEL Signal peptidase Cleavage site GFP N--C ERSS Signal peptidase Cleavage site GFP...
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