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Unformatted text preview: probability of the t-snare and v-snare will have a harder time finding eachother without the Rab. Fusion will still occur but it will be much slower. II. ER to Golgi (page 766-778) 1a) The diagram below is the primary confirmation for a hypothetical protein. Using an antibody to the protein, where would you expect it to be localized? Describe how the localization of the protein will change if we replace different portions of the protein with GFP. b) Substitution of ERSS with GFP: N--C ERSS KDEL Signal peptidase Cleavage site N--C GFP KDEL Signal peptidase Cleavage site c) Substitution of internal portion with GFP: d) Substitution of KDEL with GFP: e) For each of the proteins diagramed above (a-d), how will the protein distribution change if we neutralize the pH level in the Golgi (more basic now)? N--C ERSS KDEL Signal peptidase Cleavage site GFP N--C ERSS Signal peptidase Cleavage site GFP...
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- Spring '09