Unformatted text preview: ii. Confocal Microscopy: sharp focus with optical sections 4. Electron Microscopy a. resolution: in practical terms, .1nm (1A) b. Immunoglold can localize specific molecules c. Scanning microscopy: a 3d image of the surface of a speciman d. Multiple images can be combined to increase resolution 5. Visualizing Molecules in Living Cells a. uses light microscopy b. Strategies to introduce Membrane-impermeant molecules into cells c. “caged” Molecules d. Fluorescent Proteins can be used to Tag Individual Proteins e. Light Can be Used to Manipulate microscopic Objects...
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- Spring '09
- Confocal microscopy, specific molecules, Electron Microscopy a., Tag Individual Proteins