Lecture17.2010 - Cell-Cell Adhesion and Cell Junctions...

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Cell-Cell Adhesion and Cell Junctions Lecture 17 3/25/10 Reading: Alberts Chapter 19, pgs. 1131-1163 . Cell-cell interactions and Connections Interactions of Cells with Other Cells I. Little known about mechanisms that generate complex 3-D cell arrangements in developing organs -presume to depend heavily on selective interactions between cells of II. Experiments demonstrating cell adhesion A. Remove developing organ from chick or amphibian embryo, dissociate to 1. Disaggregate cells from 2 different developing organs, mix - form mixed clump then sort out 2. Once separated into homogeneous cluster , cells often differentiate i nto structures they would have formed within intact embryo B. Add labeled cells to dishes with monolayers of varied cell types -> cells stick to own type better III. Four distinct families of membrane proteins are most important in mediating cell-cell adhesion A. Selectins B. Certain members of immunoglobulin superfamily (IgSF) C. Certain members of integrin family D. Cadherins IV . Selectins - integral glycoprotein family ; binds to specific carbohydrate groups in oligosaccharides on cell surface ; name comes from lectins, compounds that bind specific carbohydrate groups A. Has small cytoplasmic domain , a membrane-spanning domain extracellular segment that consists of a number of separate domains (outermost domain acts as lectin) B. 3 known types E-selectin (endothelial cells); P-selectin (platelets, endothelial cells); L-selectin (present on all types of leukocytes or white blood cells). C. All recognize similar grouping of 4 sugars present on ends of certain carbohydrate chains on glycoproteins. All require calcium for binding to their carbohydrate ligands. D. Mediate transient interactions between circulating leukocytes at V. Immunoglobulin Superfamily - large family of proteins with similar domains ; most present on lymphocyte surfaces as integral proteins 1
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A. Most IgSF members
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Lecture17.2010 - Cell-Cell Adhesion and Cell Junctions...

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