MicroBio Reveiw Sheet Prelim I - TheySleep,TheyGrow,TheyDie

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They Sleep, They Grow, They Die All about the growth rate o Control Time: by limiting growth rate or limit time you are willing to expose foods Control growth rate by intrinsic and extrinsic factors intrinsic o low pH, high pH (they grow at pH 7) o low salt, high salt extrinsic o low temp, high temp. o low oxygen (may promote growth), high oxygen microbial load o low inoculums, high inoculums (promote growth) o high endogenous, low endogenous (promote growth) Sauerkraut o harvested, shredded, then salted water activity is decreased and gram negative is  suppressed lactic acid bacteria is promoted Thermus Aquaticus aerobic, thermophilic, gram negative Biotechnology paradigm raw materials biotransformation bioreactors product recovery PCR o heat denature template, allow primers to anneal (amplify) o add DNA polymerase to extend, nucleotides are incorparted (extend) o denature (high temp), anneal (low temp), extend(high temp)- all controlled by  temperature o Maxim-Gilbert vs. Sanger (standard) uses dideoxynucleotide (ddCTP), deoxy lacks oxygen, terminates chain, ‘C’ o Starter Culture single strain, mutiple, mixed o The human microbiome contains roughly 100 times as many genes as does the genome
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Craig Venter Mycoplasma genitalium o smallest genome of a free-living organism o gets along with 458 genes Biobricks o promoters, regulators, enzymes o mix and match to create artificial operons Sequencing of genomes has become the method of choice for biological investigations Facts: o Estimated 25-250 million cases of food-borne illness annually 
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This note was uploaded on 04/12/2010 for the course FDSC 394 taught by Professor Batt during the Fall '09 term at Cornell University (Engineering School).

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MicroBio Reveiw Sheet Prelim I - TheySleep,TheyGrow,TheyDie

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