Chapter 3 - Observing Microorganisms through a Microscope...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Observing Microorganisms through a Microscope Chapter 3
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Basic techniques needed to study Bacteria 1. Grow Bacteria 2. Isolate Bacteria 3. Grow Bacteria in pure culture 4. Observe Bacteria 5. Identify Bacteria
Background image of page 2
Microscope Resolving Power - ability to distinguish two distinct points absolute limit of the Resolving Power is about 1/2 the wavelength of light that is used to illuminate the specimen
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Preparing smears for staining 1. Bacteria on slide 2. Air Dry 3. Bacteria are HEAT FIXED to the slide 4. Stain is applied
Background image of page 4
Staining Reaction Stains - salts composed of a positive and negative ion, one of which is colored (chromophore) Basic Dyes - chromophore is the positive ion dye+ Cl- Acid Dyes - chromophore is the negative ion Na+ dye-
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacteria are slightly negative, so are attracted to the positive chromophore of the BASIC DYE Common Basic Dyes crystal violet methylene blue safranin basic fuchsin
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 24

Chapter 3 - Observing Microorganisms through a Microscope...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online