Chapter 5 - Microbial Metabolism Chapter 5 Metabolism all...

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Microbial Metabolism Chapter 5
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Metabolism - all of the chemical reactions within a living organism 1. Catabolism ( Catabolic ) breakdown of complex organic molecules into simpler compounds releases ENERGY 2. Anabolism ( Anabolic ) the building of complex organic molecules from simpler ones requires ENERGY
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Enzymes - catalysts that speed up and direct chemical reactions A. Enzymes are substrate specific Lipases Lipids Sucrases Sucrose Ureases Urea Proteases Proteins DNases DNA
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Enzyme Specificity can be explained by the Lock and Key Theory E + S -----> ES ------> E + P
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Naming of Enzymes - most are named by adding “ase” to the substrate Sucrose Sucrase Lipids Lipase DNA DNase Proteins Protease removes a Hydrogen Dehydrogenase removes a phosphate phosphotase
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Naming of Enzymes Grouped based on type of reaction they catalyze 1. Oxidoreductases 2. Hydrolases hydrolysis 3. Ligases synthesis
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Enzyme Components 2 Parts 1. Apoenzyme - protein portion 2. Coenzyme (cofactor) - non-protein Holoenzyme - whole enzyme
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Coenzymes Many are derived from vitamins 1. Niacin NAD (Nicotinamide adenine dinucleotide) 2. Riboflavin FAD (Flavin adenine dinucleotide) 3. Pantothenic Acid CoEnzyme A
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Factors that Influence Enzymatic Activity Denaturation of an Active Protein
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Inhibitors can effect enzymatic activity 1. Competitive Inhibitors 2. Noncompetitive Inhibitors
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This note was uploaded on 04/13/2010 for the course BIO 3302 taught by Professor Dr.nasreenhaque during the Fall '09 term at CUNY City Tech.

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Chapter 5 - Microbial Metabolism Chapter 5 Metabolism all...

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