Chapter 8 - Microbial Genetics Chapter 8 Structure and...

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Microbial Genetics Chapter 8
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Structure and Function of Genetic Material DNA & RNA DNA deoxyribonucleic acid RNA ribonucleic acid Nucleotides Phosphate group Pentose sugar Nitrogenous base
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Structure of DNA Double stranded (double helix) polymers of nucleotides 5’ to 3’ (strands are anti-parallel) Complimentary Base Pairing A - T G - C
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DNA Replication Bacteria have only 1 chromosome (ccDNA) E. coli about 4 million base pairs 1 mm long (1000 times longer than the cell) DNA takes up only about 10 % of cells volume
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E. coli DNA 1u 1,360u 1ft. 1,360 ft.
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DNA Replication occurs at the Replication Fork (5’ to 3’) DNA Helicase 1. Leading Strand ( 5’ to 3’ ) DNA Polymerase 2. Lagging Strand ( 5’ to 3’) RNA Polymerase (RNA Primer) DNA Polymerase (extends primer and digests RNA) DNA Ligase Okazaki Fragments
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Bidirectional replication Origin of replication Termination site
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Protein Synthesis DNA mRNA Protein Transcription Translation Central Dogma of Molecular Genetics
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