Biochem_exam_1_Spring_2005

Biochem_exam_1_Spring_2005 - Exam 1 Spring 05 1 Which of...

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Exam 1 – Spring 05 1. Which of the following amino acids would be most difficult to separate electrophoretically (at any pH)? a. lys, val, thr b. leu, ala, met c. his, arg, glu d. leu, lys, asp e. phe, arg, lys 2. Imagine 50mL of 0.1M NaOH is mixed with 51mL of 0.1M HCl. Which of the following values will be closest to the pH of this mixture? a. 10 b. 8 c. 7 d. 3 e. 1 3. Under certain conditions, the amino acid glycine is used as a buffer. At which of the following pH values would glycine be MOST effective as a buffer? a. 1.5 b. 2.5 c. 5.5 d. 7.0 e. 11.5 4. If duplex DNA is melted, the first regions to separate are richest in which of the following base pairings? a. AT b. CG c. UT d. GG e. UG 5. Which of the following would violate the First Law of Thermodynamics? a. the firefly converting chemical energy into light energy b. electrical propagation of the nerve impulse c. loss of total energy in the universe due to planetary motion d. eventual burning up of the sun e. a waterfall that flows upward 6. In the Meselson/Stahl experiment, after four generations of replication, what percentage of duplex DNA molecules will contain a single N-15 and N-14 labeled strand? a. 6.25 b. 12.5 c. 18.75 d. 25 e. 100 7. The assignment of an optically active amino acid stereoisomer as either D or L is based on a convention using which of the following substances? a. glyceraldehyde b. glycine c. alanine d. glucose e. 1-chloro-2-bromo-3-fluoro-4-iodomethane 2
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8. The side chains of which pair of amino acids can form a hydrophobic interaction? a. arg,val b. leu, met c. his, cys d. val, asp e. asp,asn 9. The enzymes responsible for the synthesis of DNA are called: a. extendases b. phosphatases c. intertases d. polymerases e. nucleases 10. Which of the following is not true of gel permeation chromatography? a.
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Biochem_exam_1_Spring_2005 - Exam 1 Spring 05 1 Which of...

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