Old Test - in class review on 2-11-09

Old Test - in class review on 2-11-09 - 1 The use of LiAlH...

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1. The use of LiAlH, to determine the C-terminus of a protein involves: a. Reduction- CORRECT b. An oxidation c. Denaturation d. A decarboxylation e. Esterification 2. An amino acid contains at least two functional groups: a carboxylic acid and an amine. Condensation of an amino group and a carboxyl group with loss of water forms a: a. Amide 3. Three buffers are made by combining a 1 M solution o f acetic acid with a 1M solution of sodium acetate in the ratios shown below. 1M acetic acid 1M sodium acetate Buffer 1: 10ml 90mL Buffer 2: 50ml 50ml Buffer 3: 90ml 10mL Which of the statement is true of the resulting buffers? PH of buffer 1 > pH of buffer 2 > pH of buffer 3 (CORRECT) 4. Which of the following statement bout aromatic amino acid is correct? a. Compared to tyrosine, tryptophan absorbs more ultraviolet light because the double bonds in the R group are more extensively conjugated. TRUE b. Major contribution to the characteristic absorption of light at 280nm by protein is the phenylaline R grup. FALSE. c. Histidine’s ring structure results in its being catergorized as aromatic or basic, dpending on pH. d. The prsenence of ring structure in its R group determines wheter an amino acid is aromatic or not. 5. Which are the smaller digestion products for the following peptide, when using only chemical, cyanogens bromide (CNBr) for peptide hydrolysis? H2N-Arg-Ala-Met-Lys-Lys-Met-Gly-Thr-Ile-Trp-Met-Ser-Arg-Lys-COOH(always the carboxyl side on the amino acid when cyanogens take place
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a. H2N-Arg-Ala-Met; Lys-Lys-Met; Gly-Thr-Ile-Trp-Met; Ser-Arg-Lys; COOH (CORRECT) 6. The alpha a-carbon atom of amino acid; a. Has four function groups attached. (CORRECT) and is the asymmetric center of
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This note was uploaded on 04/14/2010 for the course BHC 3023 taught by Professor Harmen during the Spring '10 term at University of South Florida.

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Old Test - in class review on 2-11-09 - 1 The use of LiAlH...

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