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Review+question+for+biochem[1]

Review+question+for+biochem[1] - Review for Exam 1 1 The...

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Review for Exam 1 1. The use of LiAlH, to determine the C-terminus of a protein involves: a. Reduction- CORRECT b. An oxidation c. Denaturation d. A decarboxylation e. Esterification 2. An amino acid contains at least two functional groups: a carboxylic acid and an amine. Condensation of an amino group and a carboxyl group with loss of water forms a: a. Amide 3. Three buffers are made by combining a 1 M solution o f acetic acid with a 1M solution of sodium acetate in the ratios shown below. 1M acetic acid 1M sodium acetate Buffer 1: 10ml 90mL Buffer 2: 50ml 50ml Buffer 3: 90ml 10mL Which of the statement is true of the resulting buffers? PH of buffer 1 > pH of buffer 2 > pH of buffer 3 (CORRECT) 4. Which of the following statement bout aromatic amino acid is correct?
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5. Which are the smaller digestion products for the following peptide, when using only chemical, cyanogens bromide (CNBr) for peptide hydrolysis? H2N-Arg-Ala-Met-Lys-Lys-Met-Gly-Thr-Ile-Trp-Met-Ser-Arg-Lys-COOH(always the carboxyl side on the amino acid when cyanogens take place a. H2N-Arg-Ala-Met; Lys-Lys-Met; Gly-Thr-Ile-Trp-Met; Ser-Arg-Lys; COOH (CORRECT) 6. The alpha a-carbon atom of amino acid; a. Has four function groups attached. (CORRECT) and is the asymmetric center of amino acid (CORRECT) i. NOT part of the planar and rigid structure of the peptide bond. ii. Is not an ionizable grup with a unique pK. iii. Cannot readily modified posttranslationally.
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