Exam 2 Spring 09 - Exam 2 1 Which of the following...

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Exam 2 1. Which of the following sequences of duplex DNA will be most resistant to melting? a. ATATCGAA TATAGCTT b. AAATCGAA TTTAGCTT c. CCATTTTG GGTAAAAC d. CCCGGATG GGGCCTAC e. TTCTTGAA AACAACTT 2. The parent compound for the correct naming of the stereoisomers of alpha amino acids found in proteins is: a. Alpha glycerol b. D-glycerol c. L-glycerol d. Alpha D-glyceraldehyde e. L-glyceraldehyde 3. Which one of the following is not among the six internationally accepted classes of enzymes? a. Hydrolases b. Ligases c. Oxidoreductases d. Polymerases e. Transferases 4. The role of an enzyme in an enzyme-catalyzed reaction is to: a. Bind a transition state intermediate, such that it cannot be converted back to substrate. b. Ensure that all of the substrate is converted to product. c. Ensure that the product is more stable than the substrate. d. Increase the rate at which substrate is converted into product. e. Make the free-energy change for the reaction more favorable.
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QUESTIONS (5-9) REFER TO THE EXPERIMENTAL DATA BELOW : You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] V 0 In Absence of Inhibitor V 0 In Presence of Inhibitor 0.0001 33 7 0.0002 50 25 0.0005 71 31 0.001 83 36 0.002 91 42 0.005 96 47 0.01 98 48 0.02 99 49 0.05 100 50 0.1 100 50 0.2 100 50 5. What is the V max in the absence of inhibitor? (Use Table) a. 50 b.100 c. 91 d. 99 e. 83 6. What is the K m in the absence of inhibitor? (Use Table) a. 0.02 b. 0.005 c. 0.01 d. 0.0002 e. 0.1 7. When [S] = 0.0004, what will V 0 be in the absence of inhibitor? (Use Table)
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Exam 2 Spring 09 - Exam 2 1 Which of the following...

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