Biochem_Exam_3_Spring_05_Answer_Key

Biochem_Exam_3_Spring_05_Answer_Key - Exam 3 Spring 05 1....

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Exam 3 Spring 05 1. Which of the following figures represents the effect of an allosteric inhibitor? a. A b. B c. C d. D e. none of the above 2. Which of the following statements is true for iodoacetamide? I. it is a reversible enzyme inhibitor II it is an irreversible enzyme inhibitor III. it reacts with sulfhydryl groups IV it reacts with the amino acid serine V. it is an uncompetitive inhibitor a. I, III and V b. II, IV and V c. I, IV and V d. I, II and V e. II and III [substrate] mM 20 40 60 80 100 120 realtive rate 2.0e-1 4.0e-1 6.0e-1 8.0e-1 1.0e+0 1.2e+0 [substrate] mM 20 40 60 80 100 120 realtive rate 2.0e-1 4.0e-1 6.0e-1 8.0e-1 1.0e+0 1.2e+0 [substrate] mM 20 40 60 80 100 relative rate 5e-2 1e-1 2e-1 2e-1 no inhibitor with inhibitor [substrate] mM 20 40 60 80 100 relative rate 5e-2 1e-1 2e-1 2e-1 A B D C 3. In the determination of kinetic constants for an enzyme that obeys Michaelis- Menten kinetics, the intercept on the x-axis of a Lineweaver-Burke plot was -4.5mM. What is the K m for this enzyme? a.-4.5 mM b. 0.5 mM c.-9/2 mM d. 4.5 mM e. 0.22 mM 4. In glycolysis, the class of enzymes that are generally not reversible are: a. isomerases b. dehydrogenases c. aldolases d. kinases e. mutases 5. Which of the following statements is NOT TRUE? a. enzymes are highly specific in binding chiral substrates b. enzymes are highly specific in catalyzing their reaction c. the forces that bind substrates to enzymes are the same as those that are responsible for correct protein folding d. the specificity of an enzyme for its substrate arises from their geometrically complementary shape e. None of the above (all of the above are true) 6. Which of the following is true for the interaction of PALA with ATCase? I. it stabilizes the R state II. it stabilizes the T state III. it acts as an inhibitor IV. it acts as a substrate V. it is an analog of the transition state a. I only b. I and IV c. I, III and V d. I and III e. I, IV and V 7. Enolase is inhibited by fluoride ions. Which of the following intermediates in glycolysis will NOT accumulate if a tissue undergoing glycolysis is treated with NaF? a. glucose 6-phosphate b. fructose 1,6-diphosphate c. pyruvate d. 1,3-phosphoglyceric acid e. 2 phosphoglyceric acid 8. In the derivation of the Michaelis-Menten equation, the steady-state assumption refers to the condition that: (BEST ANSWER) a. the rate of increase of product concentration with time is steady b. the reaction conditions (pH, ionic strength, temperature, etc.) are steady the reaction conditions (pH, ionic strength, temperature, etc....
View Full Document

Page1 / 8

Biochem_Exam_3_Spring_05_Answer_Key - Exam 3 Spring 05 1....

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online