Test_3_Practice_Problem_Answers_1

Test_3_Practice_Problem_Answers_1 - + Answer: 0.13V 3. In a...

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Test 3 practice problems 1. Given the following reaction calculate the redox potential and the free energy (ΔG °’). The Faraday constant is 96,494 J·V -1 ·mol -1 . NAD + + FADH 2 ↔ NADH + H + + FAD + NAD + + H + + 2e ↔ NADH Eº’ = -0.32V FAD + + 2H + + 2e ↔ FADH 2 Eº’ = -0.22V Answer: Redox potential = -0.01V ∆G = 19,298 Joules/mol 2. What is the reduction potential for the following reaction? Use the table in your book on page 573 to find the half reactions. Pyruvate + NADH + H + ↔ lactate + NAD
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Unformatted text preview: + Answer: 0.13V 3. In a Linweaver/Burk plot the intercept on the 1/S axis is at -2.6mM-1. What is the Km? Answer: Km = 0.38 4. What is the turnover number for a reaction that has Vmax = 1.8*10 8 Mmin-1 and E T = 10.0M? What are the units of Kcat? Answer: Kcat = 300,000 s-1 5. A particular enzyme has a Vmax = 200 and Km = 2.0. In the presence of an inhibitor the Vmax = 100 and the Km = 1.0. What type of inhibition is this? Answer: Uncompetitive Inhibition...
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